Ukrainian authorities have revoked the operating certificate of Ukraine Air Alliance, following a fatal landing accident involving an Antonov An-12 in Lviv.
The state aviation administration says the order to "terminate" the certificate was effective from 5 October.
It has not given a specific reason for the decision but it comes just after the 4 October accident which killed five of the eight occupants of the An-12.
The aircraft came down short of the runway at Lviv as it prepared to make a refuelling stop en route from Vigo to Istanbul.
Ukraine Air Alliance no longer appears on the state administration's register of operators, which was revised on 7 October.
European Union Aviation Safety Agency third-country operator approval for the carrier has also been withdrawn, according to the agency's newly-updated register of authorised carriers.
Ukrainian regulators have embarked on a technical assistance programme, in co-operation with ICAO, intended to improve certification and flight operations oversight within the country.